NutriSelect® prime Tryptic soy agar with polysorbate 80 and lecithin are suitable for the isolation and cultivation of a wide range of microorganisms surviving after treatment of surfaces and materials with antiseptics and for the enumeration and detection of aerobic mesophilic bacteria (Microbial Limit Test) from cosmetic products containing preservatives.

Appearance (clearness): clear

Appearance (color): yellowish-brown

pH-value (25 °C): 7.1 - 7.5

Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): 10 - 100

Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003)): 10 - 100

Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): 10 - 100

Inoculum on reference medium (Streptococcus pyogenes ATCC 21059): 10 - 100

Inoculum on reference medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)): 10 - 100

Inoculum on reference medium (Pseudomonas aeruginosa ATCC 10145 (WDCM 00024)): 10 - 100

Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)): 10 - 100

Inoculum on reference medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): 10 - 100

Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032)):

Colony count (Bacillus subtilis ATCC 6633 (WDCM 00003)):

Colony count (Escherichia coli ATCC 8739 (WDCM 00012)):

Colony count (Streptococcus pyogenes ATCC 21059):

Colony count (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)):

Colony count (Pseudomonas aeruginosa ATCC 10145 (WDCM 00024)):

Colony count (Candida albicans ATCC 10231 (WDCM 00054)):

Colony count (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)):

Recovery on test medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): ≥ 70 %

Recovery on test medium (Bacillus subtilis ATCC 6633 (WDCM 00003)): ≥ 70 %

Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 70 %

Recovery on test medium (Streptococcus pyogenes ATCC 21059): ≥ 70 %

Recovery on test medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)): ≥ 70 %

Recovery on test medium (Pseudomonas aeruginosa ATCC 10145 (WDCM 00024)): ≥ 70 %

Recovery on test medium (Candida albicans ATCC 10231 (WDCM 00054)): ≥ 70 %

Recovery on test medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): ≥ 50 %

Test for neutralizing capacity (Staphylococcus aureus ATCC 6538 (WDCM 00032)): passes test

Test for neutralizing capacity (Candida albicans ATCC 10231 (WDCM 00054)): passes test

Incubation: 24 hrs.; 30-35 °C; aerobic;

C.albicans and A. brasiliensis up to 5 days.

Reference: blood-agar; for C.albicans and A. brasiliensis sabouraud 2% dextrose-agar; for the test for neutralizing capacity tryptic soy agar.