TRIZMA(R) BASE, PRIMARY STANDARD AND BU& 25 G

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TRIZMA(R) BASE, PRIMARY STANDARD AND BU& 25 G

TRIZMA(R) BASE, PRIMARY STANDARD AND BU& 25 G

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TRIZMA(R) BASE, PRIMARY STANDARD AND BU& 25 G

Brands: SIGMA-ALDRICH

Rp 669.000

Tris(hydroxymethyl)aminomethane, commonly known as Trometamol, Tris base, or Trizma^® base, serves a pivotal role in diverse research applications as a biological buffer. Its optimal pKa of 8.1 makes it a preferred choice for formulating buffers like Tris-acetate-EDTA (TAE) and Tris-borate-EDTA (TBE), ensuring the maintenance of pH within the physiological range (pH 7 - 9), applicable to a wide spectrum of living organisms. Despite its utility, researchers need to exercise caution in protein studies, as Tris has the potential to interfere with the activity of specific enzymes.

SKU: T1503-25G
Categories: Reagent Chemical, pH Meter & Water Checker
Tags: REAGENT CHEMICAL, SIGMA-ALDRICH, pH Meter & Water Checker
Availability: In stock

Description

Product Name

Trizma^® base, Primary Standard and Buffer, ≥99.9% (titration), crystalline

Quality Level

description

aminopeptidase substrate

Assay

≥99.9% (titration)

form

crystalline

storage condition

dry at room temperature

technique(s)

ELISA: suitable
protein extraction: suitable

color

white

pH

10.5-12

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

methanol: soluble 26 mg/mL at 25 °C
ethylene glycol: soluble 79.1 mg/mL at 25 °C
water: soluble (678 g/l at 20 °C)

absorption

≤0.05 at 290 nm at 40%

suitability

suitable for Western blot
suitable for electrophoresis

application(s)

cell analysis
diagnostic assay manufacturing
life science and biopharma

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N