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Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm.
Product Name
Thiazolyl Blue Tetrazolium Bromide, 98%
Quality Level
Assay
98%
form
powder
technique(s)
titration: suitable
color
dark yellow
mp
195 °C (dec.) (lit.)
solubility
2-methoxyethanol: soluble 20 mg/mL
ethanol: soluble 20 mg/mL
H2O: 5 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
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