GranuCult® Oxford Agar (Base) is a selective agar for the isolation and detection of Listeria monocytogenes in milk and milk products and other food products.

Base tested with Oxford-Listeria-selektiv-supplement art. no. 1.07006.

Appearance (clearness): clear

Appearance (color): blueish-brown to greenish-brown

pH-value (25 °C): 6.8 - 7.2

Growth promotion test in accordance with the current version of DIN EN ISO 11133.

Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)):

Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)):

Inoculum on reference medium (Listeria innocua ATCC 33090 (WDCM 00017)):

Colony count (Listeria monocytogenes ATCC 35152 (WDCM 00109)):

Colony count (Listeria monocytogenes ATCC 13932 (WDCM 00021)):

Colony count (Listeria innocua ATCC 33090 (WDCM 00017)):

Recovery on test medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): ≥ 50

Recovery on test medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≥ 50

Recovery on test medium (Listeria innocua ATCC 33090 (WDCM 00017)): ≥ 50

Growth Escherichia coliATCC 25922 (WDCM 00013): total inhibition

Growth(Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition

Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): partial inhibition

Growth (Candida albicans ATCC 10231 (WDCM 00054)): partial inhibition

Black zone (Listeria monocytogenes ATCC 35152 (WDCM 00109)): black zone

Black zone (Listeria monocytogenes ATCC 13932 (WDCM 00021)): black zone

Black zone (Listeria innocua ATCC 33090 (WDCM 00017)): black zone

Incubation: up to 48 hours at 35 ± 1 °C, aerobic

A recovery rate of 50 % is equivalent to a productivity value of 0.5.

The indicated colony counts result from the sum of a triple determination.

Reference media: Tryptic Soy Agar