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Appearance (clearness) turbid with sediment

Appearance (color) green

pH-value (25 °C) 7.8 - 8.2

Growth promotion test in accordance with the current version of DIN EN ISO 11133.

Mixed inoculum of Salmonella, E.coli and P.aeruginosa

Additionaly monoculture of E.coli and E.faecalis

Confirmation on XLD agar resp. Tryptic soy agar

Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)) ≤ 100

Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)) ≤ 100

Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)) ≥ 10000

Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)) ≥ 10000

Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)) ≥ 10000

Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)) ≥ 10000

Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)) ≥ 10000

Growth on XLD agar (Salmonella typhimurium ATCC 14028 (WDCM 00031)) > 10 colonies

Growth on XLD agar (Salmonella enteritidis ATCC 13076 (WDCM 00030)) > 10 colonies

Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)) < 100 colonies

Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)) < 100 colonies

Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)) < 10 colonies

Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)) < 10 colonies

Incubation:

MKTTn broth: 24 ± 3 hours at 37 ± 1 °C aerobic

Confirmation on XLD agar resp. Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C aerobic