L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Assay (perchloric acid titration, calculated on dried substance): 99.0 - 101.0 %

Identity (IR-spectrum): passes test

Appearance: white to almost white, fine powder

Appearance of solution (25 g/l; CO₂-free water): clear and colorless

Spec. rotation (α 20/D, 100 g/l, hydrochloric acid 2 mol/l, calc. on dried substance): +31,5 to +33,0

Chloride (Cl): ≤ 200 ppm

Sulfate (SO₄): ≤ 200 ppm

Heavy metals (as Pb): ≤ 10 ppm

As (Arsenic): ≤ 5 ppm

Ca (Calcium): ≤ 10 ppm

Co (Cobalt): ≤ 5 ppm

Fe (Iron): ≤ 5 ppm

K (Potassium): ≤ 5 ppm

Mg (Magnesium): ≤ 5 ppm

Na (Sodium): ≤ 50 ppm

Zn (Zinc): ≤ 5 ppm

Ninhydrin-positive substances (LC)(any ninhydrin-positive impurity): ≤ 0.3 %

Ninhydrin-positive substances (LC) (ammonium (570 nm)): ≤ 0.1 %

Ninhydrin-positive substances (LC)(total impurities): ≤ 0.5 %

Loss on drying (105 °C; 3 h): ≤ 0.3 %