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Imidazole, also known as Glyoxalin, is an aromatic heterocyclic diazole and a key biological buffer with a pH range of 6.2 to 7.8 at 25°C. It has a wide range of applications in molecular biology, biochemistry, and proteomics. In proteomics, imidazole is essential for eluting histidine-tagged proteins from IMAC columns.
grade
Molecular Biology
Quality Level
vapor pressure
<1 mmHg ( 20 °C)
Assay
≥99% (titration)
form
crystalline powder
storage condition
dry at room temperature
technique(s)
electrophoretic mobility shift assay: suitable
molecular cloning: suitable
protein purification: suitable
color
white
pH
6.2-7.8 (25 °C)
pKa (25 °C)
6.95
Rp 12.364.000
Rp 3.098.000
Rp 1.534.000
Rp 3.250.000
Rp 2.197.000
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