The new MultiScreenHTS vacuum manifold provides enhanced performance for use with MultiScreenHTS 96- and 384-well filter plates as well as the Solvinert family of filter plates. It can be easily configured for both flow-to-waste and analyte collection. For applications that require filtrate collection for further analysis, the MultiScreenHTS vacuum manifold and filter plates offer a significant improvement in reliability over current systems. When used with MultiScreenHTS filter plates and ANSI/SBS standard receiver plates, the unique plate and manifold designs combine to eliminate gaps between the flow directors and receiver wells and provide a plate-on-plate processing option for assays where filtrate collection is required. Direct stacking also makes vacuum initiations effortless. Complete filtration assay cycles can be done with no manual intervention.