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Specifications

Appearance (clearness) clear

Typical composition (per vial): D-Cycloserine 200 mg; 4-Methylumbelliferylphosphate di sodium salt 50 mg.

Growth promotion test in accordance with ISO 11133.

Supplement tested with base Art. 1.11972.

Inoculum on reference medium

- Clostridium perfringens ATCC 10543

10 - 100

- Clostridium perfringens ATCC 13124 (WDCM 00007) 10 - 100

- Clostridium perfringens ATCC 12916 (WDCM 00080) 10 - 100

- Clostridium tetani ATCC 19406 > 10⁴

- Clostridium novyi ATCC 17861 > 10⁴

- Pseudomonas aeruginosa ATCC 27853 (WDCM 00025) > 10⁴

- Bacillus cereus ATCC 11778 (WDCM 00001) > 10⁴

- Escherichia coli ATCC 25922 (WDCM 00013) > 10⁴

- Escherichia coli ATCC 8739 (WDCM 00012) > 10⁴

Recovery on test medium

- Clostridium perfringens ATCC 10543

≥ 70 %

- Clostridium perfringens ATCC 13124 (WDCM 00007) ≥ 70 %

- Clostridium perfringens ATCC 12916 (WDCM 00080) ≥ 70 %

Growth

- Clostridium tetani ATCC 19406

none to fair

- Clostridium novyi ATCC 17861 none to fair

- Pseudomonas aeruginosa ATCC 27853 (WDCM 00025) none to poor

- Bacillus cereus ATCC 11778 (WDCM 00001) none to fair

- Escherichia coli ATCC 25922 (WDCM 00013) inhibited

- Escherichia coli ATCC 8739 (WDCM 00012) inhibited

Blacking

- Clostridium perfringens ATCC 10543

+

- Clostridium perfringens ATCC 13124 (WDCM 00007) +

- Clostridium perfringens ATCC 12916 (WDCM 00080) +

- Clostridium tetani ATCC 19406 -

- Clostridium novyi ATCC 17861 -

- Pseudomonas aeruginosa ATCC 27853 (WDCM 00025) -

- Bacillus cereus ATCC 11778 (WDCM 00001) -

- Escherichia coli ATCC 25922 (WDCM 00013) -

- Escherichia coli ATCC 8739 (WDCM 00012) -

Fluorescence

- Clostridium perfringens ATCC 10543

+

- Clostridium perfringens ATCC 13124 (WDCM 00007) +

- Clostridium perfringens ATCC 12916 (WDCM 00080) +

Incubation: 20 hrs. 37 °C.

Reference: TSC-agar for l.perfingens; Blood-agar for all other strains.