Chromocult® Coliform Agar is a selective agar suitable for the simultaneous detection and colony count of total coliforms and E. coli in foods. This medium enables E. coli and coliforms from food matrices such as raw ground beef, raw ground chicken, and raw milk to be detected, differentiated, and enumerated within 24 hours.

Appearance (clearness): clear

pH-value (25 °C): 6.8 - 7.2

Recovery on test medium (Escherichia coli ATCC 11775 (WDCM 00090)): ≥ 70 %

Recovery on test medium (Citrobacter freundii ATCC 8090): ≥ 70 %

Recovery on test medium (Enterobacter aerogenes ATCC 13048): ≥ 70 %

Recovery on test medium (Proteus mirabilis ATCC 29906 (WDCM 00023)):

Growth (Aeromonas hydrophila ATCC 7966 (WDCM 00063)): inhibited

Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): inhibited

Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): inhibited

Growth (Bacillus subtilis ATCC 6633 (WDCM 00003)): inhibited

Colony colour (Escherichia coli ATCC 11775 (WDCM 00090)): blue

Colony colour (Citrobacter freundii ATCC 8090): pink-red

Colony colour (Enterobacter aerogenes ATCC 13048): pink-red

Incubation: 24 hrs.; 35 °C; aerobic.

Reference: Tryptic soy agar.

Inhibited strains tested with three-loop-streak.